Dynamics of growth of microbial cell titer of Bradyrhizobium japonicum and Sinorhizobium fredii strains during submerged cultivation in a laboratory fermenter. A.I. SOROKINA, M.V. YAKIMENKO, S.A. BEGUN

Abstract

Dynamics of growth of microbial cell titer of Bradyrhizobium japonicum and Sinorhizobium fredii strains during submerged cultivation in a laboratory fermenter. A.I. SOROKINA, M.V. YAKIMENKO, S.A. BEGUN (Federal Scientific Center “All-Russian Research Institute of Soybeans”, Blagoveshchensk).

The results of studies of the dynamics of growth of bacterial cells of Bradyrhizobium japonicum (Jordan, 1982) and Sinorhizobium fredii (Scholla, Elkan, 1984) strains in liquid culture for 24, 48, 72 hours of submerged cultivation, assimilating a wide range of carbon sources, are presented. The most technological in ability of accumulation high titer CFU/ml in conditions of submerged cultivation are the strains of B. japonicum TM-455, BM-88, TM-469, BM-91 and S. fredii OB-46, 071, TB-496, SB-38. The maximum titer of active cells of B. japonicum CM-42 and S. fredii ББ-49 strains was obtained during 72-hour cultivation in a laboratory TN-50L series fermenter, at a cultivation temperature +28 ℃, stirrer rotation at 70 rpm and pH_medium 6.73–6.77, which amounted to 8 ∙ 10⁹ CFU/ml and 17 ∙ 10⁹ CFU/ml, respectively.